The use of Bacteriophage in Releasing two Decarboxylases from Escherichia Coli B

Two-step resistance by Escherichia coli B to bacteriophage T2.

Numerous authors have noted the difficulty in obtaining mutants of E. coli B that are resistant to bacteriophage T2 using standard procedures of plating large numbers of cells in the presence of excess phage. Yet, T2-resistant mutants appear in continuous culture at rates in consistent with this difficulty. This paradoxical result derives from the fact that resistance to T2 usually arises as a ...

Isolation of the bacteriophage lambda receptor from Escherichia coli.

A factor which inactivates the phage lambda can be extracted from Escherichia coli. This factor is a protein and is located in the outer membrane of the bacterial envelope. It is found in extracts of strains which are sensitive to phage lambda, but not in extracts of strains specifically resistant to this phage. We conclude that this factor is the lambda receptor, responsible for the specific a...

In Vitro Biocontrol of Escherichia coli Through the Immobilization of its Specific Lytic Bacteriophage on Cellulose Acetate Biodegradable Film

Background and Aims: Bacteriophages are mandatory bacterial parasites that are harmless to human and animal, which are used by dipping or spraying in food as natural antimicrobial agents. The use of these methods leads to wasting or trapping of phage in food, but its immobilization on the polymer surface facilitates the contact of phage with the host cell at the food surface. Therefore, the aim...

Bacteriophage T7 endonuclease. I. Properties of the enzyme purified from T7 phage-infected Escherichia coli B.

An endonuclease activity present in extracts from Escherichia coli B infected with T7f phage has been purified 2500fold. This enzyme, T7 endonuclease I, is the product of gene 3, and its activity is maximal 7.5 min after infection at 37”. The activity is at least 100 times greater with single stranded DNA as substrate than with duplex DNA. The limit product obtained by digestion of single stran...

The Infection of Sub-cellular Escherichia Coli, Strain B, with a Dna Preparation from T2 Bacteriophage.